Chemistry and DNA barcoding of macrolichen genus lobaria at upper montane of Crocker Range
Lichens are self-supporting symbiotic association formed by a fungus and one or several alga or cyanobacteria. The lichen symbiosis have been highly successful with more than 18,000 currently accepted species of lichen forming fungi and an estimated diversity of more than 28,000 species. Genus Lobar...
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| フォーマット: | 学位論文 |
| 言語: | 英語 英語 |
| 出版事項: |
2018
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| オンライン･アクセス: | https://eprints.ums.edu.my/id/eprint/43279/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/43279/2/FULLTEXT.pdf |
| 要約: | Lichens are self-supporting symbiotic association formed by a fungus and one or several alga or cyanobacteria. The lichen symbiosis have been highly successful with more than 18,000 currently accepted species of lichen forming fungi and an estimated diversity of more than 28,000 species. Genus Lobaria consists of a number of closely related taxa which are morphologically very similar. In Sabah, the total of 10 species of Lobaria has been described for the past 28 years where the specimens were collected from Mount Kinabalu and Crocker Range.The objectives of this research are 1) to perform the chemical profiling of Lobaria population in Sabah, and 2) to establish the DNA barcoding of Lobaria species in Sabah. A total of 41 specimens of Lobaria were sampled from Mesilau, Mount Alab and Mount Minduk Sirung. Specimens were identified based on their morphological features and chemical characters as well as DNA barcoding.All specimens were extracted using acetone and detailed chemical profiling was carried out based on 1) Thin Layer Chromatography (TLC), 2) High Performance Liquid Chromatography (HPLC), and 3) LCMS IT-TOF-MS. Lobaria specimens were identified into four different species which are L. discolor, L. pseudoretigera and new records for Sabah which are Lobaria sp. 1 and Lobaria sp. 2. From the chemical profiling, L. discolor contain lecanoric acid, gyrophoric acid and 4-O-methylgyrophoric acid while L.pseudoretigera contain retigeric acid A. Lobaria sp. 1 contain constictic acid, stictic acid, lecanoric acid and gyrophoric acid, Lobaria sp. 2 contain constictic acid, stictic acid and retigeric acid B. DNA barcoding database was established in BOLD Systems (http://www.boldsystems.org). The information on identifiers, taxonomy, specimen details, photographs, geography, and collection details were uploaded to the database. The population of Lobaria in this study has contributed to 23% of the total number of Lobaria specimens available in BOLD database. |
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