Detection of genetic polymorphism in Sabah rice using random amplified polymorphic dna (RAPD) markers

• मुख्य लेखक: Alvina Sumeetah Simon
• स्वरूप: थीसिस
• भाषा: अंग्रेज़ी; अंग्रेज़ी
• प्रकाशित: 2018
• विषय: QP501-801 Animal biochemistry
• ऑनलाइन पहुंच: https://eprints.ums.edu.my/id/eprint/44031/1/24%20PAGES..pdf; https://eprints.ums.edu.my/id/eprint/44031/2/FULLTEXT..pdf

Genetic marker is a useful tool for assessing genetic polymorphisms and diversity. The Random Amplified Polymorphic DNA (RAPD) markers are one of the markers utilized due to its ability to determine the level of genetic polymorphism, similarity, distance and phylogenetic relationship. Rice is one of the main crops in Malaysia with its production concentrating on two types of rice i.e., the irrigated and upland rice. Unfortunately, despite its importance to Malaysia's agricultural industry, information on genetic polymorphisms of the irrigated and upland rice cultivars is limited. Hence, the objective of this study is to assess the genetic polymorphisms focusing on Sabah rice using RAPD markers. Twenty-nine rice samples consist of 24 irrigated rice and five upland rice cultivars were selected for analysis. From the obtained result, a total of 101 alleles were amplified using nine random primers (OPA-01, OPA-02, OPA-03, OPA-05, OPF-09, OPK-12, OPG-17, OPG-18, OPG-19) and 94% of the amplified alleles showed polymorphisms. Two alleles named as OPA-05-B2 and OPA-01-Bll were found to be unique in upland rice cultivars and it was postulated that these alleles might be involved in upland environmental adaptation. The phylogenetic tree analysis grouped 29 rice samples into two main cluster, namely Cluster 1 and Cluster II. In addition to that, an intermediate lineage was found between Cluster I and Cluster II. Most of the irrigated rice (22 out of the 24 samples) were grouped together under Cluster I, while most of upland rice were grouped together under Cluster II. The intermediate lineage was represented by some of the upland rice as well. Based on the phylogenetic tree classification, it can be suggested that upland rice cultivar of Sabah is genetically different from the irrigated rice cultivar of Sabah. Besides that, the RAPD markers used were able to classify the different rice cultivars into distinct subgroups. The results obtained from this study provides additional insight into the genetic relationships and diversity of Sabah rice.