Detection of agrobacterium tumefaciens in artificially inoculated tomato plants using polymerase chain reaction

• المؤلف الرئيسي: Nirrcy Salimin
• التنسيق: أطروحة
• اللغة: الإنجليزية; الإنجليزية
• منشور في: 2015
• الموضوعات: SB119-124 Propagation Including breeding, selection, grafting, air layering
• الوصول للمادة أونلاين: https://eprints.ums.edu.my/id/eprint/44204/2/ABSTRACT.pdf; https://eprints.ums.edu.my/id/eprint/44204/5/FULLTEXT..pdf

Agrobacterium tumefaciens is used in plant biotechnology for its DNA transmission capabilities. It enables the insertion of foreign genes into plants, making it a widely used approach in genetic engineering producing genetically modified plants. This study aims to assess of the risk of infection posed by accidental release of the plant transgenic vector Agrobacterium tumefaciens LBA4404 into the soil inside a transgenic containment. A known Fl phenotype Solanum /ycopersicum infected by 10-5, 10-6, 10-7 and 10-8 of dilution factor of Agrobacterium tumefaciens LBA4404 containing plasmid vector pCAMBIA1303 was used for this purpose. The leaf samples of Solanum /ycopersicum were collected on the thirtieth, sixtieth and ninetieth day after the transplantation. The DNA from the leaves of Solanum lycopersicum extracted by using CTAB DNA extraction and tested for the presence of the 35s DNA promoter by using the Polymerase Chain Reaction. Testing the presence of the 35s DNA promoter by PCR revealed that none of the leave sample infected by either one of the dosages of Agrobacterium tumefaciens. Increasing the concentration and wounding the Solanum lycopersicum are the possible ways to enhance the transformation efficiency of Agrobacterium tumefaciens. This finding will be useful for the risk assessment of research that creating GM plant by using plant transgenic vector Agrobacterium tumefaciens LBA4404.