Purification and partial characterization of glutathione s-transferases from Pseudomonas sp. UW4 / Christina Kong Wen Hui
Glutathione transferases (GSTs) obtained from University of Waterloo, Canada, were purified from Pseudomonas sp. UW4, by glutathione-affinity chromatography, identified through bioinformatic analysis and their substrate specificities were investigated. SDS-polyacrylamide gel electrophoresis revealed...
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| 格式: | Thesis |
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2019
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| _version_ | 1849736042366631936 |
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| author | Christina Kong , Wen Hui |
| author_facet | Christina Kong , Wen Hui |
| author_sort | Christina Kong , Wen Hui |
| description | Glutathione transferases (GSTs) obtained from University of Waterloo, Canada, were purified from Pseudomonas sp. UW4, by glutathione-affinity chromatography, identified through bioinformatic analysis and their substrate specificities were investigated. SDS-polyacrylamide gel electrophoresis revealed that the GST purified using Sulfobromophthalein-glutahione (BSP) affinity column resolved into a single band with low molecular weight (MW) of 17 kDa. Isoselectrofocusing showed it exists in single band with pI value of 6.1. Purified GST was reactive towards ethacrynic acid, 1-chloro-2,4-dinitrobenzene, cumene hydroxide and hydrogen peroxide, but no detectable activity with trans-2-octenal, hepta-2,4-dienal and trans-4-phenyl-3-butene-2-one. This demonstrated that putative GST possessed peroxidase activity but is not involved in lipid peroxidation. The purified GST suggested to be similar to PputUW4_00801 (putative glutathione S-transferase) of Pseudomonas sp. UW4.
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| format | Thesis |
| id | oai:studentsrepo.um.edu.my:15143 |
| institution | Universiti Malaya |
| publishDate | 2019 |
| record_format | eprints |
| spelling | oai:studentsrepo.um.edu.my:151432024-10-08T17:55:17Z Purification and partial characterization of glutathione s-transferases from Pseudomonas sp. UW4 / Christina Kong Wen Hui Christina Kong , Wen Hui Q Science (General) QA Mathematics Glutathione transferases (GSTs) obtained from University of Waterloo, Canada, were purified from Pseudomonas sp. UW4, by glutathione-affinity chromatography, identified through bioinformatic analysis and their substrate specificities were investigated. SDS-polyacrylamide gel electrophoresis revealed that the GST purified using Sulfobromophthalein-glutahione (BSP) affinity column resolved into a single band with low molecular weight (MW) of 17 kDa. Isoselectrofocusing showed it exists in single band with pI value of 6.1. Purified GST was reactive towards ethacrynic acid, 1-chloro-2,4-dinitrobenzene, cumene hydroxide and hydrogen peroxide, but no detectable activity with trans-2-octenal, hepta-2,4-dienal and trans-4-phenyl-3-butene-2-one. This demonstrated that putative GST possessed peroxidase activity but is not involved in lipid peroxidation. The purified GST suggested to be similar to PputUW4_00801 (putative glutathione S-transferase) of Pseudomonas sp. UW4. 2019-07 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/15143/1/Christina_Kong.pdf application/pdf http://studentsrepo.um.edu.my/15143/2/Christina_Kong.pdf Christina Kong , Wen Hui (2019) Purification and partial characterization of glutathione s-transferases from Pseudomonas sp. UW4 / Christina Kong Wen Hui. Masters thesis, Universiti Malaya. http://studentsrepo.um.edu.my/15143/ |
| spellingShingle | Q Science (General) QA Mathematics Christina Kong , Wen Hui Purification and partial characterization of glutathione s-transferases from Pseudomonas sp. UW4 / Christina Kong Wen Hui |
| title | Purification and partial characterization of glutathione s-transferases from Pseudomonas sp. UW4 / Christina Kong Wen Hui |
| title_full | Purification and partial characterization of glutathione s-transferases from Pseudomonas sp. UW4 / Christina Kong Wen Hui |
| title_fullStr | Purification and partial characterization of glutathione s-transferases from Pseudomonas sp. UW4 / Christina Kong Wen Hui |
| title_full_unstemmed | Purification and partial characterization of glutathione s-transferases from Pseudomonas sp. UW4 / Christina Kong Wen Hui |
| title_short | Purification and partial characterization of glutathione s-transferases from Pseudomonas sp. UW4 / Christina Kong Wen Hui |
| title_sort | purification and partial characterization of glutathione s transferases from pseudomonas sp uw4 christina kong wen hui |
| topic | Q Science (General) QA Mathematics |
| url-record | http://studentsrepo.um.edu.my/15143/ |
| work_keys_str_mv | AT christinakongwenhui purificationandpartialcharacterizationofglutathionestransferasesfrompseudomonasspuw4christinakongwenhui |