Three-dimensional carbon interdigitated ring array with nanofibers as biosensor for dopamine neurotransmitter / Elyana Kosri

• Main Author: Elyana , Kosri
• Format: Thesis
• Published: 2024
• Subjects: RA Public aspects of medicine; TA Engineering (General). Civil engineering (General)

Dopamine (DA) is one of the crucial neurotransmitters in the central nervous system and is closely linked to various health disorders, whether DA is present in high or low levels, for example Parkinson's disease, which profoundly affects the health of those afflicted. This thesis presents a three-dimensional carbon interdigitated ring array with nanofibers integration (3D C-IDRA NF) for neurotransmitter detection using amperometric method. The methodologies begin with the fabrication of two-dimensional and three-dimensional carbon interdigitated electrode array with nanofibers integration (2D C-IDEA NF and 3D C-IDEA NF) using far-field electrospinning and carbon-microelectromechanical system (C-MEMS) techniques. The 3D C-IDEA NF enlectrode demonstrates better redox cycling results when evaluated using cyclic voltammetry (CV) of KCl-K3Fe(CN)6 solution compared to the 2D C-IDEA NF electrode. Consequently, the 3D C-IDEA NF electrode design is adapted to fabricate the circular ring array, which are the three-dimensional carbon interdigitated ring array with nanofibers integration (3D C-IDRA NF) and 3D C-IDRA electrodes for neurotransmitter sensing using CV and chronoamperometry (CA). The integration of porous carbon nanofibers (CNFs) on the surface of the 3D C-IDRA electrode marks a significant novel approach, enhancing the 3D C-IDRA NF electrode’s surface area, resulting in increased current peaks during CV of DA and overall improved electrode performance compared to the stand-alone 3D C-IDRA electrode. This is evident in the redox amplification factor of 2.94 and the remarkable collection efficiency of 81.1%. These outcomes highlight the advantages of combining porous CNFs with 3D C-IDRA, resulting in a superior 3D C-IDRA NF electrode for electrochemical biosensors for detecting neurotransmitters such as DA.