| 總結: | Boesenbergia rotunda is a medicinal plant belongs to the family of Zingiberaceae which
contains major compounds that exhibits pharmaceutical activities. In this study, the
methods of plant growth regulators and hairy roots transformation for in-vitro root
culture induction in B. rotunda were established. Regenerated roots morphology and
compounds accumulation in in-vitro roots and hairy roots induced were also analyzed.
Media containing either NAA alone or in combinations with BAP at different
concentrations (0, 0.5, 1.0, 2.0 mg/ mL and 0, 0.5, 1.0, 1.5, 2.0 mg/ mL of NAA and
BAP, respectively) were used to initiate in-vitro roots. The highest number of roots was
16.5 ± 3.4 per explant, when 0.5 mg/ mL NAA was used. For hairy roots transformation,
B. rotunda apical meristem was infected using A. rhizogenes strain A4. The effects of
infection times (20 mins and 1 hr), co-cultivation periods (1, 2, 3 days) and
acetosyringone (AS) on the transformation were investigated. Based on PCR,
amplification of rolC gene analysis, infection times of 20 mins to 1 hr followed by
2 days of co-cultivation period in the presence of 10 5M AS in the cultivation media
resulted in 33.3% of transformation efficiency. The profile and content of compounds in
in-vitro and hairy roots were evaluated using HPLC analysis. HPLC peaks of alpinetin,
pinocembrin, cardamonin, pinostrobin and panduratin A were identified and quantified.
In-vitro roots induced from plant growth regulators exhibited higher bioactive
compounds content as compared to controls and hairy roots induced via transformation.
Supplementation of 0.5 mg/ mL NAA in MS media showed higher accumulation of
alpinetin, pinocembrin, cardamonin and panduratin A, while combination of BAP and
NAA with similar concentration (0.5 mg/ mL) showed higher pinostrobin content as
compared to control. It is also found that exogenous PGR, enhanced the production of
cardamonin contents as compared to natural rhizome.
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