| 要約: | Plumeria rubra Linn is locally known in Malaysia as “kemboja” and belongs to family
Apocynaceae. It has been used traditionally in Southeast Asia for the treatment of malaria,
diabetes, analgesic to relieve fever and alleviate arthritis. The Thin Layer
Chromatography (TLC) profile of PR ̶ HE, PR ̶ CE, PR ̶ ME and PR ̶ WE extracts of
showed the presence of essential oils, phenols, flavonoids and terpenoids. The analysis
red flowers extracts with LCMS/MS displayed that it contains 3 ̶ caffeyolquinic acid, 5 ̶
caffeoquinic acid, 1,3 ̶ dicaffeoquinic acid, chlorogenic acid, citric acid, 3,3 ̶ di ̶ O ̶
methylellagic acid, kaempferol ̶ 3 ̶ O ̶ glucoside, kaempferol ̶ 3 ̶ rutinoside, kaempferol,
quercetin 3 ̶ O ̶ α ̶ L ̶ arabinopyranoside, quercetin, quinic acid and rutin. The TPC and
TFC contents were highest in PR-ME extract at 184.632 mg GAE/g and 203.2.2 mg QE/g,
respectively. In the six different antioxidant activity assays, PR ̶ ME extract exhibited the
best DPPH scavenging activity at IC50 value at 59.385 μg/ml, highest reducing power
toward ferric ions which was 4.950 mmol Fe2+/g extract, highest metal chelating activity
at IC50 value at 185.559 μg/mL, highest in hydrogen peroxide scavenging activity at IC50
value at 248.376μg/mL, highest in nitric oxide scavenging activity at IC50 value at 62.096
μg/mL and also highest in superoxide radical scavenging activity at IC50 value at 54.773
μg/mL. The result of XO inhibitory activity in vitro assay revealed that the PR ̶ ME extract
possesses highest inhibition effects with the IC50 value was 23.91 μg/mL. The acute
toxicity test among the males and females rats demonstrated the PR ̶ ME extract were safe
up to a maximum dose of 4000 mg/kg body weight due to no mortality and sign of toxicity
occurred within two weeks of experiment. Administration of PR ̶ ME extract at doses of
400 and 200 mg/kg to the rats significantly reduced serum uric acid level by 67.5 % and
28.9 %, respectively when compared to gout control group. Furthermore, the significant inhibitory actions on the XO activity in serum were showed at doses at 400 and 200 mg/kg
in which were 7.18 mU/mL by 43.77 % and 8.73 mU/mL by 31.64 %, respectively. The
result of in vivo study indicated that serum XO activity was correlated with liver XO
activity. XO activity in liver was significantly inhibited by PR ̶ ME extract at doses of
400 and 200 mg/kg, which were 7.76 mU/mL by 31.64 % and 9.88 mU/ml by 16.06 %,
respectively. However, PR ̶ ME extract did not show any XO inhibition effects in normal
rats. Moreover, no significant difference of body weight between treated rats and nontreated
rats within 7 days of extract administration. In conclusion, PR ̶ ME extract from
P. rubra red flower contained active phytochemical compounds as detected in LCMS/MS
has contributed to inhibition of XO activity in in vitro and in vivo which also had partly
mediated to the urate lowering effects in serum as well as the potential to scavenged free
radical intermediates and superoxide byproducts.
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