Species-specific eDNA markers for rapid detection of invasive fishes in Tasik Raban, Malaysia
| المؤلف الرئيسي: | |
|---|---|
| التنسيق: | أطروحة |
| اللغة: | الإنجليزية |
| منشور في: |
2023
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| الموضوعات: | |
| الوصول للمادة أونلاين: | https://ir.upsi.edu.my/detailsg.php?det=11144 |
| Abstract | Abstract here |
| _version_ | 1855626237516447744 |
|---|---|
| author | Nurul Fizatul Nabilah Osman |
| author_facet | Nurul Fizatul Nabilah Osman |
| author_sort | Nurul Fizatul Nabilah Osman |
| description | |
| format | Thesis |
| id | upsi-11144 |
| institution | Universiti Pendidikan Sultan Idris |
| language | English |
| publishDate | 2023 |
| record_format | sWADAH |
| record_pdf | Restricted |
| spelling | upsi-111442024-07-23 Species-specific eDNA markers for rapid detection of invasive fishes in Tasik Raban, Malaysia 2023 Nurul Fizatul Nabilah Osman TD Environmental technology. Sanitary engineering <p>The objective of this study is to develop species-specific environmental DNA (eDNA)</p><p>markers for Cichla spp. based on mitochondrial cytochrome oxidase I (COI) gene</p><p>sequences. Validation of the developed species-specific eDNA markers were done exsitu</p><p>for environmental sample from Tasik Raban. New digital PCR (dPCR) method was</p><p>also employed to detect the presence of Cichla ocellaris, and Cichla kelberi in</p><p>environmental water samples. Result from this study showed that COI gene can be used</p><p>to develop species-specific markers for species Cichla. Ex-situ validation results also</p><p>showed that the developed markers CO1 amplified specifically to specimen C. ocellaris</p><p>while CK1 amplified directly to specimen of C. kelberi. In addition, dPCR supported</p><p>the specificity analysis as absolute fluorescent quantification detected the presence of</p><p>C. ocellaris and C. kelberi eDNA in water sample at 90% detection confidence. In</p><p>conclusion, CO1 and CK1 markers were successfully developed and specific to be used</p><p>as eDNA markers for C. ocellaris and C. kelberi respectively. As an implication, eDNA</p><p>analysis with species-specific markers suitable to be used as new rapid monitoring tool</p><p>in detection of invasive species for biodiversity management and conservation</p><p>purposes.</p> 2023 thesis https://ir.upsi.edu.my/detailsg.php?det=11144 https://ir.upsi.edu.my/detailsg.php?det=11144 text eng N/A openAccess Masters Universiti Pendidikan Sultan Idris Fakulti Sains dan Matematik N/A |
| spellingShingle | TD Environmental technology. Sanitary engineering Nurul Fizatul Nabilah Osman Species-specific eDNA markers for rapid detection of invasive fishes in Tasik Raban, Malaysia |
| thesis_level | Master |
| title | Species-specific eDNA markers for rapid detection of invasive fishes in Tasik Raban, Malaysia |
| title_full | Species-specific eDNA markers for rapid detection of invasive fishes in Tasik Raban, Malaysia |
| title_fullStr | Species-specific eDNA markers for rapid detection of invasive fishes in Tasik Raban, Malaysia |
| title_full_unstemmed | Species-specific eDNA markers for rapid detection of invasive fishes in Tasik Raban, Malaysia |
| title_short | Species-specific eDNA markers for rapid detection of invasive fishes in Tasik Raban, Malaysia |
| title_sort | species specific edna markers for rapid detection of invasive fishes in tasik raban malaysia |
| topic | TD Environmental technology. Sanitary engineering |
| url | https://ir.upsi.edu.my/detailsg.php?det=11144 |
| work_keys_str_mv | AT nurulfizatulnabilahosman speciesspecificednamarkersforrapiddetectionofinvasivefishesintasikrabanmalaysia |