| _version_ |
1855626341026627584
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| author |
Siti Rukaiyah Abd Fatah
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| author_facet |
Siti Rukaiyah Abd Fatah
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| author_sort |
Siti Rukaiyah Abd Fatah
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| description |
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| format |
Thesis
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| id |
upsi-12950
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| institution |
Universiti Pendidikan Sultan Idris
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| language |
English
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| publishDate |
2019
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| record_format |
sWADAH
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| record_pdf |
Restricted
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| spelling |
upsi-129502025-07-10 Determination of the effects of different storage conditions and chemical treatments affecting protein profile and allergenicity of Cerithidea obtusa 2019 Siti Rukaiyah Abd Fatah <p>This study aimed to determine the effects of different storage conditions and chemical treatments</p><p>on protein profile and allergenicity of local edible sea snail, Cerithidea obtusa. Several snail</p><p>extracts from untreated, stored snails at different times and temperatures (4 C, 25 C and -20 C)</p><p>and chemical-treated snails (salting and vinegar) were extracted and resolved by sodium</p><p>dodecyl sulfate-polyacrylamide gel electrophoresis (SOS-PAGE) to determine their protein</p><p>profiles. Allergenic properties of all snail extracts were then determined by immunoblotting using</p><p>sera from snail allergic patients. In SOS-PAGE, the untreated extract of snail resulted in at</p><p>least 26 protein bands, between molecular weights of 8 to >250 kDa. In contrast, fewer protein</p><p>bands (in range of 5-25) were seen in almost all stored snail extracts and chemical treated</p><p>extracts, except for the frozen snails which could retain their protein profiling as the untreated</p><p>snail. Immunoblotting of the untreated snail extract identified numerous IgE-binding proteins at</p><p>various molecular weights between 25 to 245 kDa. Four major allergens at 35, 48, I 00, and 135 kDa</p><p>were detected. Immunoblotting revealed that the untreated and all the frozen snail extracts had</p><p>more allergenic proteins than the other stored snail extracts and chemical-treated extracts. The</p><p>vinegar-treated extract was demonstrated to have the least allergenic proteins. As a conclusion, C.</p><p>obtusa had four major allergens and all extracts of untreated, stored and chemical-treated snails</p><p>played a crucial role in snail allergy. These results would directly contribute to advancements in</p><p>diagnosis,management and therapeutic methods of snail-allergic patients worldwide.</p><p></p> 2019 thesis https://ir.upsi.edu.my/detailsg.php?det=12950 https://ir.upsi.edu.my/detailsg.php?det=12950 text eng N/A openAccess Masters Perpustakaan Tuanku Bainun Fakulti Sains dan Matematik N/A
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| spellingShingle |
Siti Rukaiyah Abd Fatah
Determination of the effects of different storage conditions and chemical treatments affecting protein profile and allergenicity of Cerithidea obtusa
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| thesis_level |
Master
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| title |
Determination of the effects of different storage conditions and chemical treatments affecting protein profile and allergenicity of Cerithidea obtusa
|
| title_full |
Determination of the effects of different storage conditions and chemical treatments affecting protein profile and allergenicity of Cerithidea obtusa
|
| title_fullStr |
Determination of the effects of different storage conditions and chemical treatments affecting protein profile and allergenicity of Cerithidea obtusa
|
| title_full_unstemmed |
Determination of the effects of different storage conditions and chemical treatments affecting protein profile and allergenicity of Cerithidea obtusa
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| title_short |
Determination of the effects of different storage conditions and chemical treatments affecting protein profile and allergenicity of Cerithidea obtusa
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| title_sort |
determination of the effects of different storage conditions and chemical treatments affecting protein profile and allergenicity of cerithidea obtusa
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| url |
https://ir.upsi.edu.my/detailsg.php?det=12950
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| work_keys_str_mv |
AT sitirukaiyahabdfatah determinationoftheeffectsofdifferentstorageconditionsandchemicaltreatmentsaffectingproteinprofileandallergenicityofcerithideaobtusa
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