Allergenicity determination of thermal and non-thermal treatments on Macrobrachium Rosenbergii

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Main Author: Komathi Sockalingam
Format: Thesis
Language:English
Published: 2018
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Online Access:https://ir.upsi.edu.my/detailsg.php?det=5376
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author Komathi Sockalingam
author_facet Komathi Sockalingam
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institution Universiti Pendidikan Sultan Idris
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spelling upsi-53762020-11-18 Allergenicity determination of thermal and non-thermal treatments on Macrobrachium Rosenbergii 2018 Komathi Sockalingam QL Zoology <p>This study aimed to determine the allergenicity of thermal and non-thermal</p><p>Macrobrachium rosenbergii (giant river prawn) treated extracts. This study used</p><p>experimental design. Untreated and treated protein extracts were prepared from the prawn flesh.</p><p>Prior extraction of the treated prawns, the prawn flesh were treated with thermal, non-thermal and</p><p>combination of both treatments. The prawn proteins were then analyzed using sodium dodecyl </p><p>sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to determine their protein profiling.</p><p>Allergenic proteins were detected by immunoblotting test using sera from 30 prawn-allergic patients</p><p>from Hospital Kuala Lumpur. The untreated prawns contained 27 protein fractions between 6 to 207</p><p>kDa, while the thermal-treated prawns have lesser protein bands. The major allergens were detected</p><p>at 72, 65, 48, 38, 36, and 30 kDa. Among thermal-treated prawns, steamed and boiled</p><p>elicited more protein bands compared to fried prawns. Meanwhile, among non-thermal treated</p><p>prawns, salted and freeze-treated prawns showed identical protein profiling and</p><p>allergenicity with the untreated prawn. While, vinegar-treated prawn showed less protein bands</p><p>than raw prawn with five prominent bands at 36, 30, 20, 17 and 13 kDa. Most of the protein bands in</p><p>the combined of thermal and non-thermal treated prawn extracts were disappeared. Salt boiled</p><p>extract showed bands at 36, 20 and 17 kDa, while in vinegar and boiled extract, no protein bands</p><p>were observed. The vinegar-boiled treatment showed the least allergenic bands as compared to the</p><p>other treatments in immunoblotting test. As a conclusion, this study has successfully indicated</p><p>that the combination of thermal and non-thermal treatments are the best methods to reduce the</p><p>allergenicity of M. rosenbergii. The implication of this study warrants the development of</p><p>effective diagnosis and management strategies of prawn</p><p>allergy worldwide.</p><p></p> 2018 thesis https://ir.upsi.edu.my/detailsg.php?det=5376 https://ir.upsi.edu.my/detailsg.php?det=5376 text eng N/A openAccess Masters Universiti Pendidikan Sultan Idris Fakulti Sains dan Matematik <p>Abdel Rahman, A. M., Kamath, S. D., Gagne, S., Lopata, A. L & Helleur, R. (2013).</p><p>Comprehensive proteomics approach in characterizing and quantifying allergenic proteins from</p><p>northern shrimp: Toward better occupational asthma prevention. 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spellingShingle QL Zoology
Komathi Sockalingam
Allergenicity determination of thermal and non-thermal treatments on Macrobrachium Rosenbergii
thesis_level Master
title Allergenicity determination of thermal and non-thermal treatments on Macrobrachium Rosenbergii
title_full Allergenicity determination of thermal and non-thermal treatments on Macrobrachium Rosenbergii
title_fullStr Allergenicity determination of thermal and non-thermal treatments on Macrobrachium Rosenbergii
title_full_unstemmed Allergenicity determination of thermal and non-thermal treatments on Macrobrachium Rosenbergii
title_short Allergenicity determination of thermal and non-thermal treatments on Macrobrachium Rosenbergii
title_sort allergenicity determination of thermal and non thermal treatments on macrobrachium rosenbergii
topic QL Zoology
url https://ir.upsi.edu.my/detailsg.php?det=5376
work_keys_str_mv AT komathisockalingam allergenicitydeterminationofthermalandnonthermaltreatmentsonmacrobrachiumrosenbergii