Proteomic analysis of diurnal variation in human skeletal muscle performance
| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2015
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| Online Access: | https://ir.upsi.edu.my/detailsg.php?det=772 |
| Abstract | Abstract here |
| _version_ | 1855625575360626688 |
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| author | Zulezwan Ab. Malik |
| author_facet | Zulezwan Ab. Malik |
| author_sort | Zulezwan Ab. Malik |
| description | |
| format | Thesis |
| id | upsi-772 |
| institution | Universiti Pendidikan Sultan Idris |
| language | English |
| publishDate | 2015 |
| record_format | sWADAH |
| record_pdf | Restricted |
| spelling | upsi-7722020-02-27 Proteomic analysis of diurnal variation in human skeletal muscle performance 2015 Zulezwan Ab. Malik QC Physics Phenotyping of human muscle based on its profile of myosin heavy chain isoforms is commonly used to help understand changes in muscle function. However, in many instances, measureable changes in force output or contractility occur in the absence of any change in myosin heavy chain profile. Therefore, more sophisticated analysis is required. Proteomic techniques including 2-dimensional gel electrophoresis, highperformance liquid chromatography and peptide mass spectrometry can be used to investigate changes in the abundance of hundreds of proteins simultaneously. To date, such techniques have not been used to specifically characterise the human myofibrillar proteome, or study how the myofibrillar proteome relates to muscle outputs such as peak isometric force or the velocity of contraction. This thesis presents a series of studies that develop proteomic techniques for the analysis of myofibrillar proteins as well as optimisation of techniques for measuring the range of muscle output from isometric through to velocity maximum of the human knee extensor muscles in vivo. After optimisation, the proteomic and muscle function measurement were employed to study diurnal variation. Time-of-day differences in sports performance and muscle function are widely reported, and typically, performance is 2015 thesis https://ir.upsi.edu.my/detailsg.php?det=772 https://ir.upsi.edu.my/detailsg.php?det=772 text eng N/A openAccess Doctoral Universiti Pendidikan Sultan Idris Fakulti Sains Sukan dan Kejurulatihan N/A |
| spellingShingle | QC Physics Zulezwan Ab. Malik Proteomic analysis of diurnal variation in human skeletal muscle performance |
| thesis_level | PhD |
| title | Proteomic analysis of diurnal variation in human skeletal muscle performance |
| title_full | Proteomic analysis of diurnal variation in human skeletal muscle performance |
| title_fullStr | Proteomic analysis of diurnal variation in human skeletal muscle performance |
| title_full_unstemmed | Proteomic analysis of diurnal variation in human skeletal muscle performance |
| title_short | Proteomic analysis of diurnal variation in human skeletal muscle performance |
| title_sort | proteomic analysis of diurnal variation in human skeletal muscle performance |
| topic | QC Physics |
| url | https://ir.upsi.edu.my/detailsg.php?det=772 |
| work_keys_str_mv | AT zulezwanabmalik proteomicanalysisofdiurnalvariationinhumanskeletalmuscleperformance |