Cloning And Overexpression Of Deoxyribonuclease (Nucb) From Bacillus Licheniformis In E. Coli Expression System

• Main Author: Leong , Xin Yun
• Format: Thesis
• Language: English
• Published: 2015
• Subjects: R Medicine (General)
• Online Access: http://eprints.usm.my/30479/

Deoksiribonuklease NucB daripada Bacillus licheniformis ATCC 14580 dijangka berpotensi menguraikan biofilem mikrob iaitu struktur biologi yang boleh menyebabkan banyak komplikasi buruk dalam konteks industri dan kesihatan kerana ia mempunyai 94% persamaan dengan B. licheniformis strain EI- 34-6 yang dipencil dari laut dan telah menunjukkan keupayaan untuk menguraikan biofilem yang dibentuk oleh kedua-dua golongan bakteria Gram-positif dan Gram-negatif dengan cepat. NucB deoxyribonuclease of Bacillus licheniformis ATCC 14580 is expected to disperse the microbial biofilm which is a problematic biological structure notorious for causing numerous complications in a variety of industrial and healthcare context due to its 94% similarity with that of the marine-isolated B. licheniformis strain EI-34-6 in which the latter has demonstrated the ability to rapidly break up the biofilms formed by both Gram-positive and Gram-negative bacteria.