Diversity And Characterization Of Polyhydroxyalkanoate Synthase (Phac) In Seawater And Mangrove Metagenomes

• मुख्य लेखक: Foong , Choon Pin
• स्वरूप: थीसिस
• भाषा: अंग्रेज़ी
• प्रकाशित: 2016
• विषय: QH1 Natural history (General - Including nature conservation, geographical distribution)
• ऑनलाइन पहुंच: http://eprints.usm.my/31368/

Komuniti mikrob bagi dua tanah paya bakau Pulau Pinang (Batu Maung dan Balik Pulau) yang dipengaruhi oleh aktiviti antropogenik telah dikaji dengan menggunakan pendekatan penjujukan metagenomik “shotgun” tanpa-kultur. Dua set data metagenomik (~250 GB) dihasilkan melalui platfom “Next-generation Sequencing (NGS)” Illumina HiSeq dan disimpan dalam pelayan awam “Metagenomic-Rapid Annotations using Subsystems Technology (MG-RAST)”. Analisis taksonomi mikrob menunjukkan bahawa kedua-dua tanah paya bakau Pulau Pinang didominasi oleh Bakteria (97 %), Proteobakteria (43 %) dan Deltaproteobakteria (15 %) pada peringkat domain,. filum dan kelas masing-masing. Pada peringkat genus, kebanyakan bakteria anaerobik diperhatikan terdiri daripada Deltaproteobakteria. Sebahagian besar daripada jujukan adalah milik spesis mikrob (70 %) dan filum (32 %) yang belum dikenalpasti atau belum dikultur. The microbial communities of two local Penang mangrove soils (Batu Maung and Balik Pulau) which are under anthropogenic influences were investigated using culture-independent shotgun metagenome sequencing approach. Two metagenome data sets (~250 GB) were generated from the Illumina HiSeq next-generation sequencing (NGS) platform and then deposited in Metagenomic-Rapid Annotations using Subsystems Technology (MG-RAST) public server. Microbial taxonomic analysis showed that both Penang mangrove soils were dominated by Bacteria (97 %), Proteobacteria (43 %) and Deltaproteobacteria (15 %) at the domain, phylum and class levels, respectively. At the genus level, predominance of anaerobic bacteria was observed and mostly belonged to Deltaproteobacteria. A large portion of the reads belonged to unknown or yet uncultured microbial species (70 %) and microbial phyla (32 %).