Construction Of Vectors For The Overexpression Of Recombinant Human Growth Hormone In Bacillus Megaterium

• Main Author: Rashid, Norhashima Abd.
• Format: Thesis
• Language: English
• Published: 2009
• Subjects: QH1 Natural history (General - Including nature conservation, geographical distribution)
• Online Access: http://eprints.usm.my/41298/

Bagi mengekspreskan gen hGH di dalam Bacillus megaterium, 3 konstrak telah direka. Konstrak-konstrak ini dinamakan sebagai Construct 1 (M7hGH), Construct 2 (R2L4sphGH) dan Construct 3 (spBsubhGH). Construct 1 direka dengan memasukkan gen pengawalatur (kodon penamat, tapak perlekatan ribosom, jujukan TAACA dan kodon pemula) yang akan diekspreskan di dalam sel. Manakala Construct 2 telah dicipta untuk diekpreskan di luar sel yang mana mengandungi isyarat peptida daripada Bacillus brevis dan gen. In order to express the hGH gene in Bacillus megaterium, 3 constructs were designed. The constructs were named as Construct 1 (M7hGH), Construct 2 (R2L4sphGH) and Construct 3 (spBsubhGH). Construct 1 was designed with regulatory features [stop codon, ribosome binding site (RBS), TAACA sequence and start codon] meant for intracellular expression. Construct 2, was created to express human growth hormone (hGH) extracellularly by using the signal peptide from Bacillus brevis.