Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates
Cholera is a major health issue, affecting millions of lives annually. In light of the recurrent outbreaks of cholera, there is a pressing need for the development of vaccines that allow rapid mass vaccination. In this study, genetically modified vaccine candidates, VCUSM21P and VCUSM22P, were desig...
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| Format: | Thesis |
| Language: | English |
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2011
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| Online Access: | http://eprints.usm.my/43233/ |
| Abstract | Abstract here |
| _version_ | 1854967940487577600 |
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| author | Murugaiah, Chandrika |
| author_facet | Murugaiah, Chandrika |
| author_sort | Murugaiah, Chandrika |
| description | Cholera is a major health issue, affecting millions of lives annually. In light of the recurrent outbreaks of cholera, there is a pressing need for the development of vaccines that allow rapid mass vaccination. In this study, genetically modified vaccine candidates, VCUSM21P and VCUSM22P, were designed. VCUSM21P is a prototrophic vaccine which encodes non-toxic cholera toxin A (ctxA) subunit immunogen and has accessory cholera enterotoxin (ace), zonula occludens toxin (zot) and repeats-in-toxin C/A (rtxC/A) mutations. On the other hand, VCUSM22P is ace, zot, ctxA, rtxC/A and haemagglutinin/protease (hap) mutant. Both mutants were found not to disassemble the actin of HEp2 cells. |
| first_indexed | 2025-10-17T08:21:20Z |
| format | Thesis |
| id | usm-43233 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2025-10-17T08:21:20Z |
| publishDate | 2011 |
| record_format | eprints |
| record_pdf | Abstract |
| spelling | usm-432332019-04-12T05:26:31Z http://eprints.usm.my/43233/ Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates Murugaiah, Chandrika R5-920 Medicine (General) Cholera is a major health issue, affecting millions of lives annually. In light of the recurrent outbreaks of cholera, there is a pressing need for the development of vaccines that allow rapid mass vaccination. In this study, genetically modified vaccine candidates, VCUSM21P and VCUSM22P, were designed. VCUSM21P is a prototrophic vaccine which encodes non-toxic cholera toxin A (ctxA) subunit immunogen and has accessory cholera enterotoxin (ace), zonula occludens toxin (zot) and repeats-in-toxin C/A (rtxC/A) mutations. On the other hand, VCUSM22P is ace, zot, ctxA, rtxC/A and haemagglutinin/protease (hap) mutant. Both mutants were found not to disassemble the actin of HEp2 cells. 2011-09 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/43233/1/CHANDRIKA%20MURUGAIAH.pdf Murugaiah, Chandrika (2011) Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates. PhD thesis, Universiti Sains Malaysia. |
| spellingShingle | R5-920 Medicine (General) Murugaiah, Chandrika Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates |
| title | Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates |
| title_full | Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates |
| title_fullStr | Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates |
| title_full_unstemmed | Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates |
| title_short | Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates |
| title_sort | construction and evaluation of multigene mutants of vibrio cholerae o139 as vaccine candidates |
| topic | R5-920 Medicine (General) |
| url | http://eprints.usm.my/43233/ |
| work_keys_str_mv | AT murugaiahchandrika constructionandevaluationofmultigenemutantsofvibriocholeraeo139asvaccinecandidates |