Immunoproteomic Analysis Of Mycobacterium Tuberculosis And Nontuberculous Mycobacteria Towards The Discovery Of Potential Universal Or Species Specific Biomarkers
The immunoproteomic analysis of whole cell protein (WCP) of Mycobacterium tuberculosis complex (MTBC) and non–tuberculous mycobacteria (NTM) was carried out to identify the potential species specific and cross genus conserved species biomarkers present in the mycobacterial species. The WCP of Mycoba...
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
2017
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/45328/ |
| Abstract | Abstract here |
| _version_ | 1855632381888692224 |
|---|---|
| author | Razman, Aimi Zafira |
| author_facet | Razman, Aimi Zafira |
| author_sort | Razman, Aimi Zafira |
| description | The immunoproteomic analysis of whole cell protein (WCP) of Mycobacterium tuberculosis complex (MTBC) and non–tuberculous mycobacteria (NTM) was carried out to identify the potential species specific and cross genus conserved species biomarkers present in the mycobacterial species. The WCP of Mycobacterium species were separated using SDS-PAGE and followed by Western blot assay to detect IgM,
IgG and IgA immune response in pooled sera of TB patients and healthy subjects as well as the individual sera. The immunoreactive bands in WB assay were divided into 4 region of antigens; (1) above 67 kDa; (2) 67 kDa – 32 kDa; (3) 30 kDa, 28kDa and 26 kDa; and (4) below 25 kDa. |
| first_indexed | 2025-10-17T08:26:49Z |
| format | Thesis |
| id | usm-45328 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2025-10-17T08:26:49Z |
| publishDate | 2017 |
| record_format | EPrints |
| record_pdf | Restricted |
| spelling | usm-453282019-09-06T02:04:18Z http://eprints.usm.my/45328/ Immunoproteomic Analysis Of Mycobacterium Tuberculosis And Nontuberculous Mycobacteria Towards The Discovery Of Potential Universal Or Species Specific Biomarkers Razman, Aimi Zafira R735-854 Medical education. Medical schools. Research The immunoproteomic analysis of whole cell protein (WCP) of Mycobacterium tuberculosis complex (MTBC) and non–tuberculous mycobacteria (NTM) was carried out to identify the potential species specific and cross genus conserved species biomarkers present in the mycobacterial species. The WCP of Mycobacterium species were separated using SDS-PAGE and followed by Western blot assay to detect IgM, IgG and IgA immune response in pooled sera of TB patients and healthy subjects as well as the individual sera. The immunoreactive bands in WB assay were divided into 4 region of antigens; (1) above 67 kDa; (2) 67 kDa – 32 kDa; (3) 30 kDa, 28kDa and 26 kDa; and (4) below 25 kDa. 2017-01 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/45328/1/AIMI%20ZAFIRA%20RAZMAN.pdf Razman, Aimi Zafira (2017) Immunoproteomic Analysis Of Mycobacterium Tuberculosis And Nontuberculous Mycobacteria Towards The Discovery Of Potential Universal Or Species Specific Biomarkers. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | R735-854 Medical education. Medical schools. Research Razman, Aimi Zafira Immunoproteomic Analysis Of Mycobacterium Tuberculosis And Nontuberculous Mycobacteria Towards The Discovery Of Potential Universal Or Species Specific Biomarkers |
| thesis_level | Master |
| title | Immunoproteomic Analysis Of Mycobacterium Tuberculosis And Nontuberculous Mycobacteria Towards The Discovery Of Potential Universal Or Species Specific Biomarkers |
| title_full | Immunoproteomic Analysis Of Mycobacterium Tuberculosis And Nontuberculous Mycobacteria Towards The Discovery Of Potential Universal Or Species Specific Biomarkers |
| title_fullStr | Immunoproteomic Analysis Of Mycobacterium Tuberculosis And Nontuberculous Mycobacteria Towards The Discovery Of Potential Universal Or Species Specific Biomarkers |
| title_full_unstemmed | Immunoproteomic Analysis Of Mycobacterium Tuberculosis And Nontuberculous Mycobacteria Towards The Discovery Of Potential Universal Or Species Specific Biomarkers |
| title_short | Immunoproteomic Analysis Of Mycobacterium Tuberculosis And Nontuberculous Mycobacteria Towards The Discovery Of Potential Universal Or Species Specific Biomarkers |
| title_sort | immunoproteomic analysis of mycobacterium tuberculosis and nontuberculous mycobacteria towards the discovery of potential universal or species specific biomarkers |
| topic | R735-854 Medical education. Medical schools. Research |
| url | http://eprints.usm.my/45328/ |
| work_keys_str_mv | AT razmanaimizafira immunoproteomicanalysisofmycobacteriumtuberculosisandnontuberculousmycobacteriatowardsthediscoveryofpotentialuniversalorspeciesspecificbiomarkers |