The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells

Show other versions (1)

Mitragyna speciosa Korth (Rubiaceae) is one of the medicinal plants used traditionally to treat various types of diseases especially in Thailand and Malaysia. Its anti-inflammatory and analgesic properties of its crude form are well documented. In this study, the cellular mechanism involved in the a...

Full description

Bibliographic Details
Main Author: Utar, Zulkhurnain
Format: Thesis
Language:English
Published: 2014
Subjects:
RK1-715 Dentistry
Online Access:http://eprints.usm.my/46473/
Abstract Abstract here
_version_ 1855632498356125696
author Utar, Zulkhurnain
author_facet Utar, Zulkhurnain
author_sort Utar, Zulkhurnain
description Mitragyna speciosa Korth (Rubiaceae) is one of the medicinal plants used traditionally to treat various types of diseases especially in Thailand and Malaysia. Its anti-inflammatory and analgesic properties of its crude form are well documented. In this study, the cellular mechanism involved in the anti-inflammatory effects of mitragynine, the major bioactive constituent, was investigated. The effects of mitragynine on the mRNA and protein expression of COX-1 and COX-2 and the production of prostaglandin E2 (PGE2) were investigated in LPS-treated RAW264.7 macrophage cells. Quantitative RT-PCR was used to assess the mRNA expression of COX-1 and COX-2. Protein expression of COX-1 and COX-2 were assessed using Western blot analysis and the level of PGE2 production was quantified using Parameter™ PGE2 Assay (R&D Systems).
first_indexed 2025-10-17T08:30:08Z
format Thesis
id usm-46473
institution Universiti Sains Malaysia
language English
last_indexed 2025-10-17T08:30:08Z
publishDate 2014
record_format EPrints
record_pdf Restricted
spelling usm-464732020-03-09T03:44:31Z http://eprints.usm.my/46473/ The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells Utar, Zulkhurnain RK1-715 Dentistry Mitragyna speciosa Korth (Rubiaceae) is one of the medicinal plants used traditionally to treat various types of diseases especially in Thailand and Malaysia. Its anti-inflammatory and analgesic properties of its crude form are well documented. In this study, the cellular mechanism involved in the anti-inflammatory effects of mitragynine, the major bioactive constituent, was investigated. The effects of mitragynine on the mRNA and protein expression of COX-1 and COX-2 and the production of prostaglandin E2 (PGE2) were investigated in LPS-treated RAW264.7 macrophage cells. Quantitative RT-PCR was used to assess the mRNA expression of COX-1 and COX-2. Protein expression of COX-1 and COX-2 were assessed using Western blot analysis and the level of PGE2 production was quantified using Parameter™ PGE2 Assay (R&D Systems). 2014-01 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/46473/1/Zulkhurnain%20Bin%20Utar24.pdf Utar, Zulkhurnain (2014) The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells. Masters thesis, Universiti Sains Malaysia.
spellingShingle RK1-715 Dentistry
Utar, Zulkhurnain
The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells
thesis_level Master
title The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells
title_full The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells
title_fullStr The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells
title_full_unstemmed The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells
title_short The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells
title_sort effects of mitragynine from mitragyna speciosa on the mrna expression of cox 1 and cox 2 in lipopolysaccharide stimulated raw264 7 macrophage cells
topic RK1-715 Dentistry
url http://eprints.usm.my/46473/
work_keys_str_mv AT utarzulkhurnain theeffectsofmitragyninefrommitragynaspeciosaonthemrnaexpressionofcox1andcox2inlipopolysaccharidestimulatedraw2647macrophagecells
AT utarzulkhurnain effectsofmitragyninefrommitragynaspeciosaonthemrnaexpressionofcox1andcox2inlipopolysaccharidestimulatedraw2647macrophagecells

Similar Items