The effect of choline based ionic liquids on antifungal, physical and mechanical properties of silicone denture liners

• Main Author: Munir, Nadia
• Format: Thesis
• Language: English
• Published: 2025
• Subjects: R Medicine; RK Dentistry
• Online Access: http://eprints.usm.my/62832/

This in vitro study aimed to develop novel choline based ionic liquids incorporated silicone denture liners (SDBSLs) with better antifungal potential and compliance with standard criteria for long term liners. The modified liners were then assessed for their antifungal activity, mechanical and physical properties. Choline salicylate and choline borate ionic liquids were synthesised and characterised with Fourier transform infrared spectroscopy and nuclear magnetic resonance for surface and purity analysis, respectively. The prepared ionic liquids were incorporated into SDBSLs (Moloplast B; DETAX Germany) at varying concentrations (1%, 2%, and 5%) to constitute two experimental groups and six subgroups; CB (CB1, CB2 & CB5); choline borate incorporated SDBSLs and CS (CS1, CS2 & CS5); choline salicylate incorporated SDBSLs. Conventional and antifungal drug; itraconazole (1.25% wt/wt), incorporated silicone denture liners (SDBSL) served as negative control and positive control. The samples were subjected to mechanical testing with Shore A hardness test through durometer. While tear strength and tensile bond strength were evaluated through universal testing machine. Physical testing was conducted to evaluate water sorption, solubility, and weight change following immersion in distilled water for 1 week and 6 weeks with weighing through an analytical balance. Data was statistically processed by SPSS version 25using ANOVA and all pairwise multiple comparison with post hoc analysis of Tukey’s test with α= 0.05 (p < 0.001). The results revealed that choline borate 2% (CB2) had a considerable inhibitory impact on C. albicans fungal growth followed by choline salicylate 2% (CS2). Choline salicylate 1% (CS1) demonstrated the maximum tear strength when compared to the positive control. The experimental groups had significantly lower mean Shore A hardness values than the positive and negative controls respectively. The negative control group had the highest tensile bond strength, followed by CS1. Following 1 week of water immersion, the positive control had the highest water sorption whereas CS2 demonstrated the lowest sorption. In contrast, the positive control displayed consistently the least solubility after 6 weeks of immersion, whereas the negative control showed the highest solubility. The drug-impregnated liners (positive control) gained the most weight and CS1 gained the least weight following 1 week of immersion. After 6 weeks of immersion, all study groups gained weight, with CB5 acquired most weight and CS1 acquired the least. The study reveals that incorporation of choline salicylate in silicone liners has significant effect on enhanced antifungal potential and compliance with mechanical and physical properties. However, further studies including dynamic viscoelastic property and cytotoxicity are needed to be investigated before the clinical trials.