Microvascular Endothelial Function In Obesity; And The Effect Of Pharmacological Intervention With Orlistat And Sibutramine On Microvascular Endothelial Function And Selected Cardiovascular Risk Markers

• Main Author: Al-Tahami, Belqes Abdullah Mohammad
• Format: Thesis
• Language: English
• Published: 2012
• Subjects: R5-920 Medicine (General)
• Online Access: http://eprints.usm.my/63421/

Obesity increases the risk of a variety of medical problems and cardiovascular diseases. Endothelial dysfunctionis one of the earliest vascular changes that occur in the pathogenesis.. Of cardiovascular diseases. This phd work is composed of three studies. The first study aimed to optimize a technique used to assess microvascular endothelial function, using laser doppler fluximetry (ldf) and the process of iontophoresis. Ldf measures skin perfusion, while iontophoresis is a non-invasive method of introducing charged substances across skin by means of a small electric current to evaluate both endotheliumdependent and endothelium independent vasodilatation. Firstly, the effect of sodium chloride used as a vehicle to dilute the iontophoresis drugs acetylcholine (ach) and sodium nitroprusside (snp) on non-specific vasodilatation was assessed. Secondly, to determine the number of electrical pulses needed to reach the maximum response during iontophoresis of ach and snp using low current strength. Thirdly, the reproducibility of different parameters during iontophoresis of ach and snp) were also determined. The initial iontophoresis protocol used consisted of 7 electrical pulses of2 minutes each separated by l minute current free interval.