| 總結: | Conventional plastics have become an essential commodity that provides comfort through their applications in the packaging, medical, agricultural, and pharmaceutical industries. However, traditional plastic is recalcitrant to microbial degradation. Bioplastics like polyhydroxyalkanoates (phas) are biodegradable and biocompatible materials that have proven to be an alternative to synthetic plastics. One of the significant problems of pha biosynthesis is the cost of carbon sources, which accounts for about 40% of production costs. The use of major food-grade oils is uneconomical and unsustainable as it may limit the availability of cooking oil and trigger an increase in the price of edible oil, leading to food-feed competition. Therefore, glycerine pitch produced pha in cupriavidus malaysiensis usmaa1020 via single-stage cultivation and optimized in a 13 l bioreactor. Two other monomers were incorporated by the addition of 1,4 butanediol and 1-pentanol, resulting in the synthesis of a terpolymer consisting of 3hb, 3hv and 4hb monomers [p(3hb-co-3hv-co-4hb)]. Effects of different concentrations of glycerine pitch, 1-pentanol, 1,4 butanediol, and oleic acid were studied.
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