| Summary: | High-risk hpv infection is the primary cause of cervical cancer, the fourth most common cancer among women. Current treatment options are limited, and recurrence rates remain high. Probiotic administration presents a promising, safe, and cost-effective strategy for managing hpv infections. The probiotic properties of lactiplantibacillus plantarum probio87 fulfilled the requirements for probiotics, including its carbohydrate utilization, acid tolerance, bile tolerance, antimicrobial ability, symbiosis properties, prebiotic utilization, mucin adhesion, haemolysis, hydrogen peroxide production, and antibiotic resistance profile. The inhibitory effects of l. Plantarum probio87's cell-free supernatant (cfs) were tested in vitro on cervical cancer cell lines using the mtt (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test, the angiogenesis assay, and the cignal finder reporter & dual-glo® luciferase assay. The results demonstrated that the cfs of l. Plantarum probio87 significantly (ρ < 0.05) inhibited the growth of hpv-mediated cervical cancer cell lines (hela and caski) compared to the negative control group. However, in c-33a (non-hpv related) cells, there was no obvious difference (ρ > 0.05). Furthermore, this probiotic inhibited the generation of solid angiogenesis promoters in all three cancer cell lines.
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