The Preparation And Characterization Of Polyhydroxybutyrate-Co-Valerate Tricalcium Phosphate Scaffolds For Bone Tissue Engineering

• Main Author: Ishak, Norfatiha
• Format: Thesis
• Language: English; English
• Published: 2019
• Subjects: R Medicine (General)
• Online Access: http://eprints.utem.edu.my/id/eprint/24682/; https://plh.utem.edu.my/cgi-bin/koha/opac-detail.pl?biblionumber=116861

Ceramic scaffolds have been widely used in biomedical application to treat bone defects. Enhancing the ceramic scaffold by using polymeric materials as coating may improve their strength however it will alter their pore characteristic as well as degradation behaviour which is crucial for the success of scaffold requirement. Polyhydroxybutyrate-co-valerate (PHBV) is degradable and has excellent compatibility properties for biomaterials. Thus the objectives of this study are (i) to determine the sintering temperature for the fabrication by using polymeric sponge method (ii) to analyse the effect of PHBV coated Tricalcium (TCP) scaffold after in-vitro immersion in simulated body fluid (SBF) solution for 16 weeks (iii) to evaluate the bioactivity and biocompatibility of the PHBV coated TCP scaffolds. Polyhydroxybutyrate-co-valerate (PHBV) coated TCP scaffolds were fabricated using polymeric sponge method. Two types of calcium phosphate (CaP) were used as matrix which are commercial (C-CP) and locally (L-TCP) supplied. The CaP scaffolds were sintered at optimised sintering temperature of 1450°C. The initial mechanical strength of the commercial and in-house TCP scaffolds are 36.64 KPa and 10.06 KPa respectively. The ceramic scaffold were then coated with PHBV using dip coating method, resulting in the increased of the compression strength to 140.00 KPa (PHBV coated C-CP) and 148.00 KPa ( PHBV coated L-TCP) respectively. The scaffold contained interconnect pores with a range of size from 200 to 400μm, and porosity within 81% to 83%. Their mechanical strength in simulated body fluid (SBF) solution was retained up to 12 weeks with good pore integrity structure. The formation of bone-like apatite in the shape of globular and cauliflower-like cluster was observed after 4 weeks of immersion in SBF solution. The presence of apatite mineral was confirmed by FTIR, XRD and EDX analysis indicating bioactive ability of the scaffold. Biocompatibility analysis shows that the scaffolds were able to retain Saos-2 cells after 24 hours indicating their ability to allow cells proliferation to adhere to it. The increasing cell metabolic activity up to day 14 was also observed suggested that the scaffold is compatible with cells and non toxic. This finding has indicated that the PHBV coated TCP scaffold is a compatible and comparable material that is potential to be used in bone tissue engineering.