Fundamental study of pulse electric field effects on hela cell cultured over extracellular matrix protein micro-patterned surface

• Main Author: Abd Rahman, Nur Adilah
• Format: Thesis
• Language: English; English
• Published: 2017
• Subjects: R Medicine (General)
• Online Access: http://eprints.uthm.edu.my/845/

Electroporation (EP) is a method of controlling cell function by using pulses of electrical fields to create pores through cell membrane and causes other substance around it to be absorbed into the cell. This method has led to a variety of medical applications, particularly in cell studies. In this study, a high voltage of 2 kV/cm with pulse duration of 30 µs was applied on HeLa cell (human cervical cancer cell) to investigate the electroporation process. In addition, this study focused on the effect of protein coated surface, combined with the pulse parameter mentioned above, to look at its effect on HeLa cell when exposed to high voltage. Thus, will lead towards cell surface attachment factors interrogation plus the presence of electric field as the stimulator for an aggressive growth rate of the cells. This was achieved by using the micro contact printing (μCP) method. The result showed positive respond on the effect of EP on protein printed surface combination where HeLa cells were grown. The 50µm was chosen as the best-pattern size for cell alignment by using fibronectin. From the cell guidance study we could clearly see the cell responses on the protein patterned surface are much elongated in comparison to the control. In addition, the cells plated on this patterned surface were further investigated with electroporation technique, in order to see the effect of electroporation on the cancer cell proliferation and other cellular activities. The result shows that the cells aligned and elongated on fibronectin pattern with PEF than without PEF exposure. The combination of these two techniques will contribute towards understanding the cell surface interface and cell surface attachment factors which may lead towards a new method for guiding cell towards wound healing process.