Identification of an unknown pigmented marine bacteria using 16S rRNA and pyoverdine gene sequence analysis
Also available in printed version : QH434 N87 2008 raf
| Auteur principal: | |
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| Autres auteurs: | |
| Format: | Bachelor thesis |
| Langue: | anglais |
| Publié: |
Universiti Teknologi Malaysia
2025
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| Sujets: | |
| Accès en ligne: | https://utmik.utm.my/handle/123456789/114108 |
| Abstract | Abstract here |
| _version_ | 1854975072469516288 |
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| author | Nur Izzati Mohd. Noh |
| author2 | Mohd. Shahir Shamsir Omar, supervisor |
| author_facet | Mohd. Shahir Shamsir Omar, supervisor Nur Izzati Mohd. Noh |
| author_sort | Nur Izzati Mohd. Noh |
| description | Also available in printed version : QH434 N87 2008 raf |
| format | Bachelor thesis |
| id | utm-123456789-114108 |
| institution | Universiti Teknologi Malaysia |
| language | English |
| publishDate | 2025 |
| publisher | Universiti Teknologi Malaysia |
| record_format | dspace |
| record_pdf | Abstract |
| spelling | utm-123456789-1141082025-08-21T00:53:31Z Identification of an unknown pigmented marine bacteria using 16S rRNA and pyoverdine gene sequence analysis Nur Izzati Mohd. Noh Mohd. Shahir Shamsir Omar, supervisor Microbial genomics Microbial growth Also available in printed version : QH434 N87 2008 raf An unknown Pseudomonas sp. pigmented marine bacteria were isolated from the mucus of 'bawal emas' fish, Trachinotus blochii. The bacteria is identified using 16s RNA and pyoverdine gene sequence analysis. Pyoverdine is the primary siderophore that forms the mechanism of fluorescence in bacteria. Using universal primers for 16sRNA (pAf and fD1) and designed primers (conserved sequence of pvcD gene of P.aeruginosa PAO1) for pvcD, the smallest gene in pyoverdine synthesis operon, these targeted sequences were amplified using PCR and identified through DNA sequencing. The amplified fragment using the designed primers was identified as beta-ketoacyl synthase-like protein of Pseudomonas putida F1 while the 16S rRNA sequence analysis identified the unknown marine bacteria as Pseudomonas putida F1. Both sequence analysis result successfully corroborate each other in identifying the unknown bacteria as Pseudomonas putida F1. However the failure of the identification for pvcD pyoverdine gene can be attributed to many factors, many of which beyond the scope of this project. Hence, future work can focus in identifying the presence of pyoverdine gene in Pseudomonas putida. snhas UTM 67 p. Project Paper (Sarjana Muda Sains (Biologi)) - Universiti Teknologi Malaysia, 2008 2025-04-18T07:25:20Z 2025-04-18T07:25:20Z 2008-05 Bachelor thesis https://utmik.utm.my/handle/123456789/114108 valet-20150507-112358 vital:77485 ENG Closed Access UTM Complete Completion Unpublished application/pdf Universiti Teknologi Malaysia |
| spellingShingle | Microbial genomics Microbial growth Nur Izzati Mohd. Noh Identification of an unknown pigmented marine bacteria using 16S rRNA and pyoverdine gene sequence analysis |
| thesis_level | Other |
| title | Identification of an unknown pigmented marine bacteria using 16S rRNA and pyoverdine gene sequence analysis |
| title_full | Identification of an unknown pigmented marine bacteria using 16S rRNA and pyoverdine gene sequence analysis |
| title_fullStr | Identification of an unknown pigmented marine bacteria using 16S rRNA and pyoverdine gene sequence analysis |
| title_full_unstemmed | Identification of an unknown pigmented marine bacteria using 16S rRNA and pyoverdine gene sequence analysis |
| title_short | Identification of an unknown pigmented marine bacteria using 16S rRNA and pyoverdine gene sequence analysis |
| title_sort | identification of an unknown pigmented marine bacteria using 16s rrna and pyoverdine gene sequence analysis |
| topic | Microbial genomics Microbial growth |
| url | https://utmik.utm.my/handle/123456789/114108 |
| work_keys_str_mv | AT nurizzatimohdnoh identificationofanunknownpigmentedmarinebacteriausing16srrnaandpyoverdinegenesequenceanalysis |