Optimization of maltogenic amylase immobilization in sodium alginate and chitosan alginate beads
Also available in printed version
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| Format: | Bachelor thesis |
| Language: | English |
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Universiti Teknologi Malaysia
2025
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| Online Access: | https://utmik.utm.my/handle/123456789/44210 |
| Abstract | Abstract here |
| _version_ | 1854934097967710209 |
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| author | Chan Hui Min |
| author2 | Nor Hasmaliana Abdul Manas, supervisor |
| author_facet | Nor Hasmaliana Abdul Manas, supervisor Chan Hui Min |
| author_sort | Chan Hui Min |
| description | Also available in printed version |
| format | Bachelor thesis |
| id | utm-123456789-44210 |
| institution | Universiti Teknologi Malaysia |
| language | English |
| publishDate | 2025 |
| publisher | Universiti Teknologi Malaysia |
| record_format | dspace |
| record_pdf | Abstract |
| spelling | utm-123456789-442102025-08-20T21:22:25Z Optimization of maltogenic amylase immobilization in sodium alginate and chitosan alginate beads Chan Hui Min Nor Hasmaliana Abdul Manas, supervisor Chemical Engineering Also available in printed version Nowadays, oligosaccharides with non-digestible properties have gained worldwide attention and have been widely used in food and pharmaceutical industries. Malto-oligosaccharides which contain reducing properties can be produced from starch as the raw material by enzymatic hydrolysis. A purified maltogenic amylase is a suitable enzyme to catalyse the production of high yields of malto-oligosaccharides. Enzyme immobilization technique is introduced to increase the enzyme stability and improve enzyme activity recovery. Hence, encapsulation immobilization technique is discussed in this study due to its high biocompatibility and prevention loss of enzymatic activity. Alginate beads were used as a support material to immobilize maltogenic amylase but there is still enzyme leaking problem due to its low mechanical strength. Chitosan serves as a coating agent which form membrane layer on alginate beads to overcome this problem for better beads stability. Optimization was done to determine the optimum concentrations of independent variables which were sodium alginate, calcium chloride and chitosan to yield the highest activity recovery response of enzyme. The activity recovery response of chitosan-alginate beads was analysed by central composite design response surface methodology (CCD RSM) and compared with sole alginate beads. In this study, addition of chitosan coating layer on alginate beads shows a small improvement of 7.86% in activity recovery but significantly improves the strength of beads. The activity recovery yield by alginate beads was 0.0445 U/mg while for chitosan-alginate beads was 0.048 U/mg. Both beads mathematical model equations were quadratic and significant. The R2 value for the sodium alginate model was 0.7751 while for the chitosan-alginate model was 0.9171. Chitosan-alginate bead shows better model compared to sodium alginate model. Therefore, chitosan is a good material to increase the activity recovery and stability of alginate beads and the models obtained in this research were confirmed. zainurin UTM 66 p. Thesis (Sarjana Muda Kejuruteraan Kimia) - Universiti Teknologi Malaysia, 2021 2025-03-11T06:12:28Z 2025-03-11T06:12:28Z 2021 Bachelor thesis https://utmik.utm.my/handle/123456789/44210 vital:144177 valet-20211121-134043 ENG Closed Access UTM Complete Unpublished Completion application/pdf Universiti Teknologi Malaysia |
| spellingShingle | Chemical Engineering Chan Hui Min Optimization of maltogenic amylase immobilization in sodium alginate and chitosan alginate beads |
| thesis_level |
Other
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| title | Optimization of maltogenic amylase immobilization in sodium alginate and chitosan alginate beads |
| title_full | Optimization of maltogenic amylase immobilization in sodium alginate and chitosan alginate beads |
| title_fullStr | Optimization of maltogenic amylase immobilization in sodium alginate and chitosan alginate beads |
| title_full_unstemmed | Optimization of maltogenic amylase immobilization in sodium alginate and chitosan alginate beads |
| title_short | Optimization of maltogenic amylase immobilization in sodium alginate and chitosan alginate beads |
| title_sort | optimization of maltogenic amylase immobilization in sodium alginate and chitosan alginate beads |
| topic | Chemical Engineering |
| url | https://utmik.utm.my/handle/123456789/44210 |
| work_keys_str_mv | AT chanhuimin optimizationofmaltogenicamylaseimmobilizationinsodiumalginateandchitosanalginatebeads |