Medium optimization for biomass production of lactobacillus reuteri using submerged cultivation
Also available in printed version
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| Format: | Bachelor thesis |
| Language: | English |
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Universiti Teknologi Malaysia
2025
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| Online Access: | https://utmik.utm.my/handle/123456789/44370 |
| Abstract | Abstract here |
| _version_ | 1854934099976781824 |
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| author | Ong Jing Han |
| author2 | Daniel Joe Dailin, supervisor |
| author_facet | Daniel Joe Dailin, supervisor Ong Jing Han |
| author_sort | Ong Jing Han |
| description | Also available in printed version |
| format | Bachelor thesis |
| id | utm-123456789-44370 |
| institution | Universiti Teknologi Malaysia |
| language | English |
| publishDate | 2025 |
| publisher | Universiti Teknologi Malaysia |
| record_format | dspace |
| record_pdf | Abstract |
| spelling | utm-123456789-443702025-08-20T21:51:16Z Medium optimization for biomass production of lactobacillus reuteri using submerged cultivation Ong Jing Han Daniel Joe Dailin, supervisor Chemical Engineering Also available in printed version Lactobacillus reuteri is a probiotic bacterium that has an exclusive metabolism pathway to transform glycerol to 1,3-propanediol (1,3-PDO) and 3-hyroxypropiomc acid (3-HP) via propanediol-utilization (Pdu) pathway. In producing these probiotic products, microbial biomass and the bioactive metabolites are often considered as raw materials in these processes. To achieve significant biomass production, double-carbon sources cultivation strategy was applied, and the composition of medium was optimized by response surface methodology. In the statistical optimization, the factors that were optimized are lactose, glucose and yeast extract respectively using Box-Behnken design. The optimized cultivation medium obtained from the design contained: 10.0 g L'1 lactose, 50.0 g L'1 glucose and 44.3434 g L'1 yeast extract, 2.0 g L'1 K2HPO4, 3.0 g L'1 CHsCOONa, 2.0 g L 1 C6H17N3O7, 0.5 g L 1 MgS04, and 0.05 g L 1 MnS04. Lastly, the growth kinetics was studied for both optimized and un-optimized media by plotting the cell growth curve with samples collected at every 2 hours interval. The cell biomass of L. reuteri produced was 3.574 g L"1 compared to 1.807 g L'1 in sole carbon-source MRS medium. The optimized double-carbon source strategy had improved biomass production of L. reuteri fermentation. zainurin UTM 49 p. Thesis (Sarjana Muda Kejuruteraan Kimia) - Universiti Teknologi Malaysia, 2021 2025-03-11T06:18:05Z 2025-03-11T06:18:05Z 2021 Bachelor thesis https://utmik.utm.my/handle/123456789/44370 vital:144621 valet-20211201-143953 ENG Closed Access UTM Complete Unpublished Completion application/pdf Universiti Teknologi Malaysia |
| spellingShingle | Chemical Engineering Ong Jing Han Medium optimization for biomass production of lactobacillus reuteri using submerged cultivation |
| thesis_level |
Other
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| title | Medium optimization for biomass production of lactobacillus reuteri using submerged cultivation |
| title_full | Medium optimization for biomass production of lactobacillus reuteri using submerged cultivation |
| title_fullStr | Medium optimization for biomass production of lactobacillus reuteri using submerged cultivation |
| title_full_unstemmed | Medium optimization for biomass production of lactobacillus reuteri using submerged cultivation |
| title_short | Medium optimization for biomass production of lactobacillus reuteri using submerged cultivation |
| title_sort | medium optimization for biomass production of lactobacillus reuteri using submerged cultivation |
| topic | Chemical Engineering |
| url | https://utmik.utm.my/handle/123456789/44370 |
| work_keys_str_mv | AT ongjinghan mediumoptimizationforbiomassproductionoflactobacillusreuteriusingsubmergedcultivation |