Optimisation of thermophilic Bacillus licheniformis strain MEA-01�s proteolytic activity towards casein based on temperature and incubation period
Also available in printed version
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| Other Authors: | |
| Format: | Bachelor thesis |
| Language: | English |
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Universiti Teknologi Malaysia
2025
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| Online Access: | https://utmik.utm.my/handle/123456789/45366 |
| Abstract | Abstract here |
| _version_ | 1854975052439617536 |
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| author | Nur Amira Syahirah Mazlan |
| author2 | Zarita Zakaria, supervisor |
| author_facet | Zarita Zakaria, supervisor Nur Amira Syahirah Mazlan |
| author_sort | Nur Amira Syahirah Mazlan |
| description | Also available in printed version |
| format | Bachelor thesis |
| id | utm-123456789-45366 |
| institution | Universiti Teknologi Malaysia |
| language | English |
| publishDate | 2025 |
| publisher | Universiti Teknologi Malaysia |
| record_format | dspace |
| record_pdf | Abstract |
| spelling | utm-123456789-453662025-08-20T19:43:15Z Optimisation of thermophilic Bacillus licheniformis strain MEA-01�s proteolytic activity towards casein based on temperature and incubation period Nur Amira Syahirah Mazlan Zarita Zakaria, supervisor Biosciences and medical engineering Also available in printed version Thermostable protease is a proteolytic enzyme that cleaves the peptide bonds in protein molecules to produce smaller peptides and free amino acids that functions at a relatively high temperature. However, the available studies about thermostable protease isolated from hotsprings in Malaysia are limited which led to insufficient information available on the optimum conditions for efficient enzymatic activity and other possible applications of the thermostable protease. In this study, the thermostable protease activity of Bacillus licheniformis strain MEA-01 towards casein based on temperature and incubation period were optimised. The B. licheniformis strain MEA-01 was cultured and centrifuged to obtain the supernatant containing thermostable protease. The supernatant or sample was first analysed for protein concentration by using the Lowry�s method. The protein concentration was 1.377 � 0.008 mg/mL. The sample then investigated for proteolytic activity by using caseinolytic enzyme assay at 37 �C for 10 minutes. The amount of tyrosine liberated was 0.732 � 0.058 �mole while the proteolytic activity of sample was 0.805 � 0.063 U/mL. Meanwhile, the optimisation of proteolytic activity based on temperature and incubation period was also analysed by using the same caseinolytic enzyme assay. The assay for optimisation of temperature was conducted for 30 minutes whereas assay for optimisation of incubation period was carried out at 37 �C. The result showed that the optimum temperature was 40 �C with 0.226 � 0.047 �mole of tyrosine and 10.824 � 2.244 U/mL of proteolytic activity; and the optimum incubation period was 10 minutes with 0.212 � 0.055 �mole of tyrosine and 30.417 � 7.899 U/mL of proteolytic activity. The casein hydrolysate obtained during the caseinolytic enzyme assay for optimisation of incubation period was further analysed for casein degradation patterns by using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The analysis showed that casein was completely degraded after 24 hours zulaihi UTM 59 p. Project Paper (Sarjana Muda Sains (Biologi Industri)) - Universiti Teknologi Malaysia, 2018 2025-03-12T04:27:05Z 2025-03-12T04:27:05Z 2018 Bachelor thesis https://utmik.utm.my/handle/123456789/45366 vital:119124 valet-20190122-10193 ENG Closed Access UTM Complete Unpublished Completion application/pdf Universiti Teknologi Malaysia |
| spellingShingle | Biosciences and medical engineering Nur Amira Syahirah Mazlan Optimisation of thermophilic Bacillus licheniformis strain MEA-01�s proteolytic activity towards casein based on temperature and incubation period |
| thesis_level | Other |
| title | Optimisation of thermophilic Bacillus licheniformis strain MEA-01�s proteolytic activity towards casein based on temperature and incubation period |
| title_full | Optimisation of thermophilic Bacillus licheniformis strain MEA-01�s proteolytic activity towards casein based on temperature and incubation period |
| title_fullStr | Optimisation of thermophilic Bacillus licheniformis strain MEA-01�s proteolytic activity towards casein based on temperature and incubation period |
| title_full_unstemmed | Optimisation of thermophilic Bacillus licheniformis strain MEA-01�s proteolytic activity towards casein based on temperature and incubation period |
| title_short | Optimisation of thermophilic Bacillus licheniformis strain MEA-01�s proteolytic activity towards casein based on temperature and incubation period |
| title_sort | optimisation of thermophilic bacillus licheniformis strain mea 01�s proteolytic activity towards casein based on temperature and incubation period |
| topic | Biosciences and medical engineering |
| url | https://utmik.utm.my/handle/123456789/45366 |
| work_keys_str_mv | AT nuramirasyahirahmazlan optimisationofthermophilicbacilluslicheniformisstrainmea01sproteolyticactivitytowardscaseinbasedontemperatureandincubationperiod |