Synthesis of oleyl oleate in a solvent free system using 'Rhizomucor miehei' lipase reinforced nanobioconjugates
Also available in printed version
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| Format: | Bachelor thesis |
| Language: | English |
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Universiti Teknologi Malaysia
2025
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| Online Access: | https://utmik.utm.my/handle/123456789/46490 |
| Abstract | Abstract here |
| _version_ | 1854975077526798336 |
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| author | Siti Adibah Yumni Md. Jailani |
| author2 | Roswanira Abdul Wahab, supervisor |
| author_facet | Roswanira Abdul Wahab, supervisor Siti Adibah Yumni Md. Jailani |
| author_sort | Siti Adibah Yumni Md. Jailani |
| description | Also available in printed version |
| format | Bachelor thesis |
| id | utm-123456789-46490 |
| institution | Universiti Teknologi Malaysia |
| language | English |
| publishDate | 2025 |
| publisher | Universiti Teknologi Malaysia |
| record_format | dspace |
| record_pdf | Abstract |
| spelling | utm-123456789-464902025-08-21T02:31:19Z Synthesis of oleyl oleate in a solvent free system using 'Rhizomucor miehei' lipase reinforced nanobioconjugates Siti Adibah Yumni Md. Jailani Roswanira Abdul Wahab, supervisor Science Also available in printed version Oleyl oleate is a substance exhibiting good fat soluble properties and wetting behavior at interfaces as well as being non-toxic. These favorable properties have resulted in oleyl oleate being commonplace in formulation of cosmetics, pharmaceutical products and lubricant industries. Nonetheless, the preferred chemical route to produce oleyl oleate uses high temperature that incidentally promote degradation of the esters as well as undesired side reactions. In this perspective, an alternative method using Rhizomucor miehei lipase (RML) immobilized onto activated chitosan-graphene oxide (CS-GO) has been proposed. The catalytic competency of the developed RML-CS-GO was evaluated against the free RML under a varying conditions of temperature, enzyme loading, molar ratio of alcohol to acid as well as half-life of the RML-CSGO for the solventless synthesis of oleyl oleate. Field Emission Scanning Microscopy (FESEM), Attenuated Total Reflectance (ATR) Spectroscopy and Thermogravimetric Analysis (TGA) revealed successful attachment of the RML onto the surface of CS-GOs. However, the results showed that lower concentrations of oleyl oleate were observed in reactions of RML-CSGO (47.3% at 40�C, 3mg/ml, molar ratio of (1:3, v/v) oleic acid and oleyl alcohol) compared to the free RML (91.3% at 30�C, 3mg/ml, molar ratio of (1:3, v/v) oleic acid and oleyl alcohol), respectively. Considering the lower yield of the ester attained in the RML-CSGO, revisions in protocols for matrix development and enzyme immobilization employed in this study may prove pertinent for developing catalytically enhanced and functional RML-CSGO fahmimoksen UTM 85 p. Project Paper (Sarjana Muda Sains (Kimia)) - Universiti Teknologi Malaysia, 2016 2025-03-12T05:05:31Z 2025-03-12T05:05:31Z 2016 Bachelor thesis https://utmik.utm.my/handle/123456789/46490 vital:122145 valet-20190312-124059 ENG Closed Access UTM Complete Unpublished Completion application/pdf Universiti Teknologi Malaysia |
| spellingShingle | Science Siti Adibah Yumni Md. Jailani Synthesis of oleyl oleate in a solvent free system using 'Rhizomucor miehei' lipase reinforced nanobioconjugates |
| thesis_level | Other |
| title | Synthesis of oleyl oleate in a solvent free system using 'Rhizomucor miehei' lipase reinforced nanobioconjugates |
| title_full | Synthesis of oleyl oleate in a solvent free system using 'Rhizomucor miehei' lipase reinforced nanobioconjugates |
| title_fullStr | Synthesis of oleyl oleate in a solvent free system using 'Rhizomucor miehei' lipase reinforced nanobioconjugates |
| title_full_unstemmed | Synthesis of oleyl oleate in a solvent free system using 'Rhizomucor miehei' lipase reinforced nanobioconjugates |
| title_short | Synthesis of oleyl oleate in a solvent free system using 'Rhizomucor miehei' lipase reinforced nanobioconjugates |
| title_sort | synthesis of oleyl oleate in a solvent free system using rhizomucor miehei lipase reinforced nanobioconjugates |
| topic | Science |
| url | https://utmik.utm.my/handle/123456789/46490 |
| work_keys_str_mv | AT sitiadibahyumnimdjailani synthesisofoleyloleateinasolventfreesystemusingrhizomucormieheilipasereinforcednanobioconjugates |