Production of violacein pigment using solid state fermentation
Also available in printed version
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| Format: | Bachelor thesis |
| Language: | English |
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Universiti Teknologi Malaysia
2025
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| Online Access: | https://utmik.utm.my/handle/123456789/52128 |
| Abstract | Abstract here |
| _version_ | 1854934083275063296 |
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| author | Khairiyah Amirah Kamarudin |
| author2 | Wan Azlina Ahmad, supervisor |
| author_facet | Wan Azlina Ahmad, supervisor Khairiyah Amirah Kamarudin |
| author_sort | Khairiyah Amirah Kamarudin |
| description | Also available in printed version |
| format | Bachelor thesis |
| id | utm-123456789-52128 |
| institution | Universiti Teknologi Malaysia |
| language | English |
| publishDate | 2025 |
| publisher | Universiti Teknologi Malaysia |
| record_format | dspace |
| record_pdf | Abstract |
| spelling | utm-123456789-521282025-08-20T17:33:18Z Production of violacein pigment using solid state fermentation Khairiyah Amirah Kamarudin Wan Azlina Ahmad, supervisor Science Also available in printed version Natural pigment from Chromobacterium violaceum, violacein is highly in demand due to its pharmacological properties. Submerged fermentation of Chromobacterium violaceum results in low yield of violacein due to extraction problems. Hence, solid state fermentation is proposed where separation of pigments is easier. Solid state fermentation of Chromobacterium violaceum UTM5 on sugarcane bagasse was tested in shake flask and a column system. Crude violacein was extracted using ethyl acetate and characterized using UV-Visible Spectrophotometer and Fourier Transform Spectroscopy (FTIR). Highest violacein production (5.11 mg/g SCB) was observed in sugarcane bagasse supplemented with 100 mg/L of L-tryptophan at 30 °C for three days. The violacein observed at 556 nm using UV-Vis spectra and within the range of ?max as reported by other researchers. FTIR spectrum of violacein showed a weak band of OH stretching at 3215.98 cm-1 which might overlap with the N-H band. Two stretching bands at 1603.98 cm-1 and 1702.85 cm-1 were assigned to the C=O amide groups. Intermediate C-N band was observed at 1203.62 cm-1. This study showed that the violacein produced in solid state fermentation was quite similar as that produced using submerged fermentation fahmimoksen UTM 50 p. Project Paper (Sarjana Muda Sains (Kimia Industri)) - Universiti Teknologi Malaysia, 2017 2025-03-14T07:14:16Z 2025-03-14T07:14:16Z 2017 Bachelor thesis https://utmik.utm.my/handle/123456789/52128 vital:111896 valet-20180624-092946 ENG Closed Access UTM Complete Unpublished Completion application/pdf Universiti Teknologi Malaysia |
| spellingShingle | Science Khairiyah Amirah Kamarudin Production of violacein pigment using solid state fermentation |
| thesis_level |
Other
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| title | Production of violacein pigment using solid state fermentation |
| title_full | Production of violacein pigment using solid state fermentation |
| title_fullStr | Production of violacein pigment using solid state fermentation |
| title_full_unstemmed | Production of violacein pigment using solid state fermentation |
| title_short | Production of violacein pigment using solid state fermentation |
| title_sort | production of violacein pigment using solid state fermentation |
| topic | Science |
| url | https://utmik.utm.my/handle/123456789/52128 |
| work_keys_str_mv | AT khairiyahamirahkamarudin productionofviolaceinpigmentusingsolidstatefermentation |