Optimization of fermentation strategy for enhanced production of thermostable xylanase by recombinant Escherichia Coli
Also available in printed version
| मुख्य लेखक: | |
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| अन्य लेखक: | |
| स्वरूप: | Doctoral thesis |
| भाषा: | अंग्रेज़ी |
| प्रकाशित: |
Universiti Teknologi Malaysia
2025
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| विषय: | |
| ऑनलाइन पहुंच: | https://utmik.utm.my/handle/123456789/57094 |
| Abstract | Abstract here |
| _version_ | 1854975085750779904 |
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| author | Wan Aizatul Nadihah Ropandi |
| author2 | Hesham A. El Enshasy, supervisor |
| author_facet | Hesham A. El Enshasy, supervisor Wan Aizatul Nadihah Ropandi |
| author_sort | Wan Aizatul Nadihah Ropandi |
| description | Also available in printed version |
| format | Doctoral thesis |
| id | utm-123456789-57094 |
| institution | Universiti Teknologi Malaysia |
| language | English |
| publishDate | 2025 |
| publisher | Universiti Teknologi Malaysia |
| record_format | dspace |
| record_pdf | Abstract |
| spelling | utm-123456789-570942025-08-21T04:47:31Z Optimization of fermentation strategy for enhanced production of thermostable xylanase by recombinant Escherichia Coli Wan Aizatul Nadihah Ropandi Hesham A. El Enshasy, supervisor Chemical Energy engineering Also available in printed version Xylan is the second most abundant polysaccharide in plant cell wall which is hydrolyzed by the group of enzymes called hemicellulase. � -1, 4 endo xylanase is considered as the most important among the xylanase enzymes, due to its wide industrial applications. Escherichia coli BL21 with a plasmid vector pET-22b (+) carrying xylanase coding gene, which was isolated from the extremely thermophilic bacterium called Thermotoga neapolitana, was used in the current study to enhance xylanase production. In phase 1 of this study, using the statistical approach called response surface methodology, the optimum media composition for enhanced xylanase production was successfully identified. Up to 800 IU mL-1 xylanase activity was observed in optimized media, which is around 3 folds higher compared to the activity achieved in unoptimized medium. In phase 2, optimization of lactose-based induction strategy was carried out to enhance the xylanase production. As a result of this induction optimization, the intracellular xylanase production was enhanced up to 2600 IU mL-1. In phase 3, as a part of process scale up, the study was focused on developing suitable fed-batch fermentation conditions, by optimizing nutrients and inducer feeding strategy. With the optimized fed batch fermentation conditions in 16 L stirred tank bioreactor, the xylanase activity was enhanced up to 11000 IU mL-1, which is 4 to 5 folds higher compared to activity reported in previous studies. During physicochemical characterization in phase 4 of the current study, the optimum temperature and pH of xylanse enzyme was found to be 80癈 and 6.5, respectively. Among the metal ions and chelating agents tested, zinc sulfate and ethylenediaminetetraacetic acid were found to have the highest inhibitory effect on xylanase enzyme in this study. zulaihi UTM 243 p. Thesis (Doktor Falsafah (Kejuruteraan Bioproses)) - Universiti Teknologi Malaysia, 2019 2025-03-17T04:35:32Z 2025-03-17T04:35:32Z 2019 Doctoral thesis https://utmik.utm.my/handle/123456789/57094 vital:131508 valet-20200123-084832 ENG Closed Access UTM Complete Completion Unpublished application/pdf Universiti Teknologi Malaysia |
| spellingShingle | Chemical Energy engineering Wan Aizatul Nadihah Ropandi Optimization of fermentation strategy for enhanced production of thermostable xylanase by recombinant Escherichia Coli |
| thesis_level | PhD |
| title | Optimization of fermentation strategy for enhanced production of thermostable xylanase by recombinant Escherichia Coli |
| title_full | Optimization of fermentation strategy for enhanced production of thermostable xylanase by recombinant Escherichia Coli |
| title_fullStr | Optimization of fermentation strategy for enhanced production of thermostable xylanase by recombinant Escherichia Coli |
| title_full_unstemmed | Optimization of fermentation strategy for enhanced production of thermostable xylanase by recombinant Escherichia Coli |
| title_short | Optimization of fermentation strategy for enhanced production of thermostable xylanase by recombinant Escherichia Coli |
| title_sort | optimization of fermentation strategy for enhanced production of thermostable xylanase by recombinant escherichia coli |
| topic | Chemical Energy engineering |
| url | https://utmik.utm.my/handle/123456789/57094 |
| work_keys_str_mv | AT wanaizatulnadihahropandi optimizationoffermentationstrategyforenhancedproductionofthermostablexylanasebyrecombinantescherichiacoli |