Effect of temperature, salinity and diet on the growth of harpacticoid copepod, Euterpina acutifrons, in laboratory
Harpacticoid copepod, Euterpina acutifrons is a potential live feed for marine fish larvae due to its high nutritional value and broad range of sizes through their various developmental stages. However, the mass production of this species remains elusive due to culture technique constraints and lack...
| 主要作者: | |
|---|---|
| 格式: | Thesis |
| 语言: | 英语 英语 |
| 出版: |
2014
|
| 主题: | |
| 在线阅读: | https://eprints.ums.edu.my/id/eprint/43848/1/24%20PAGES..pdf https://eprints.ums.edu.my/id/eprint/43848/2/FULLTEXT..pdf |
| 总结: | Harpacticoid copepod, Euterpina acutifrons is a potential live feed for marine fish larvae due to its high nutritional value and broad range of sizes through their various developmental stages. However, the mass production of this species remains elusive due to culture technique constraints and lack of knowledge of its biology and ecology. Thus, this study was conducted to provide information of the optimum temperature, salinity and suitable diet for culture of E. acutifrons in laboratory. Samples used in this study were isolated from Sepanggar Bay, Kota Kinabalu. Pure stock cultures of E. acutifrons were maintained in a temperature controlled chamber at 25±2°C with light intensity of l00μmolm-2s-1 and photoperiod 12:12 light-dark. Observation on the life cycle of E. acutifrons shows that they took 9 days-12 days to complete. To determine the optimum parameters for culture establishment, three experiments were conducted. The parameters tested were temperature (25°C, 27°C, 29°C, 31 °C), salinities, (Spsu, 10psu, 1Spsu, 20psu, 25psu, 30psu, 35psu, 40psu, 45psu) and the diets; monoalgal species Isochrysis (Iso), Tetraselmis (Tet), Chaetoceros (Cs), Nannochloropsis (Nan) and mixed species or binary algal (Iso & Tet, Iso & Cs, Iso & Nan, Tet & Cs, Tet & Nan, Cs & Nan). The results show that optimum temperature was at 27°C with a mean population of 800± 100 individuals/lS0ml and maximum growth of 0.438K, while at 31°C, all copepods were died. Optimum salinity was at 30psu with maximum growth of 0.8755K, similar to water salinity at sampling site. Population growth of E. acutifrons was generally higher when fed with binary algal diets compared to mono-algal diets. The best result was obtained from E. acutifrons fed with mixture of Cs & Iso with a final population of 1020±75 individuals/lS0ml and maximum growth of 0.463K. This could be due to content of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) in both diets which are essential nutrients for crustaceans growth and development. This work has shown that E. acutifrons has the potential to be cultivated in laboratory to meet the demands for live feed in hatcheries. |
|---|