Overlap PCR strategy and cloning of Prostate Specific Membrane Antigen (PSMA) gene in pichia pastoris yeast recombinant expression system / Nassrin Abduljalil Badroon
In this study, PSMA was divided into 3 fragments, G1, G2 and G3. Two-step PCR was done to constructed G1 sub-divided fragments (a, b, c, d, e, and f). G2 and G3 were amplified using specific primers and the obtained PCR products were subsequently purified and proceed for cloning. In order to clone...
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| 格式: | Thesis |
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2013
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